Quantitative measurements of factors influencing bacterial attachment to meat surfaces
نویسنده
چکیده
................................................................................................................................................... 1 Publications and conference presentation relevant to the thesis ........................................................... 4 Chapter 1: Literature review and study aims ........................................................................................................ 6 Chapter 2: Bacterial attachment to immobilized extracellular matrix proteins in vitro ..................................... 37 Chapter 3: Effect of pH, salt and chemical rinses on bacterial attachment to extracellular matrix proteins ..... 58 Chapter 4: Cultured C2C12 cell lines as a model for assessment of bacterial attachment to bovine primary muscle cells ........................................................................................................................................ 78 Chapter 5: Influence of soluble extracellular matrix proteins on bacterial attachment to immobilized extracellular matrix proteins .............................................................................................................. 91 Chapter 6: General discussions .......................................................................................................................... 109 References ........................................................................................................................................... 117 Appendix A ........................................................................................................................................... 132 Appendix B ........................................................................................................................................... 135
منابع مشابه
Cell surface charge characteristics and their relationship to bacterial attachment to meat surfaces.
Cell surface charge and hydrophobicity of Bacillus subtilis, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, Serratia marcescens, Staphylococcus aureus, and Staphylococcus epidermidis were determined by hydrocarbon adherence, hydrophobic interaction, and electrostatic interaction chromatography. Surface charge and hydrophobicity were compared with the initial attachmen...
متن کاملOn the Relevance of Microbial Biofilms for Persistence of Staphylococcus Aureus in Dairy Farms
Biofilms are defined as bacterial communities surrounded by a structured extracellular polymeric matrix which is adhered to biotic or abiotic surfaces [1]. Biofilm formation develops in two steps: firstly, a primary attachment occurs onto polymeric surfaces, mediated partly by cell-wall associated adhesins; secondly, cell-cell proliferation forms a multilayered biofilm, mediated by production o...
متن کاملExplanation of the Physical Factors Influencing the Formation of Place Attachment Among Newcomers: A Case Study of Elective Urban Spaces of Isfahan
Place attachment is the emotional connection that develops between a person and thephysical environment around them. After more than forty years of research in the field ofplace attachment, the cause and effect as well as the physical factors that affect it have notbeen determined. The features and specifications of a place in both physical and socialdimensions have an influence on the developm...
متن کاملAnchoring of Azospirillum brasilense to hydrophobic polystyrene and wheat roots
The anchoring (irreversible attachment) of Azospirillium brasilense Cd to hydrophobic polystyrene and to root surfaces was compared. Live A. brasilense Cd cells attached in significantly greater numbers to roots than to polystyrene, regardless of treatments made to the surfaces or to the bacterial cells. Triton X-100, Na2EDTA and several bacterial -inhibitory substances reduced bacterial attach...
متن کاملCsgA production by Escherichia coli O157:H7 alters attachment to abiotic surfaces in some growth environments.
The role of curli expression in attachment of Escherichia coli O157:H7 to glass, Teflon, and stainless steel (SS) was investigated through the creation of csgA knockout mutants in two isolates of E. coli O157:H7. Attachment assays using epifluorescence microscopy and measurements of the force of adhesion of bacterial cells to the substrates using atomic force microscopy (AFM) force mapping were...
متن کامل